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How Botox Works?

There are several types of cosmetic treatments. Botox is one of the best aesthetic alternatives to achieve a natural appearance. Herein is everything you need to know about Botox, including what it is, how this treatment works, and how long the results lasts.

Botulinum toxin treatment eliminates soft lines and wrinkles, caused by muscle contractions.The anti-wrinkle treatment also restores facial appearance by relaxing muscles along the treated site. Dynamic wrinkles areformed when certain muscles contract. Botulinum toxin treatment eliminates wrinkles and fine lines by relaxing the facial muscles. However, pregnant or lactating mothers should steer clear of botulinum toxin injections. This article delves deeper into botox.

How Does Botulinum Toxin Work?

Botulinum toxin is harmful once released in huge amounts. According to Cracowski and Rustic (2020), botulinum toxin attacks the nervous system cells that release acetylcholine. The anti-wrinkle treatment prevents muscle activity. Jimsheleishvili& Marwaha (2022) explained that acetylcholine binds to certain sites on a protein known as SNARE. This protein forms complex compounds between the muscle and the nerve cells. SNARE makes the nerves contract, hindering muscle movement.

Acetylcholine selectively invades one of the proteins and divides it into two portions. The invasion of one protein is enough to interfere completely with its roles. However, there are a lot of speculations on how acetylcholine realises and cleaves its SNAREs targets. This is because each of the other forms of botulinum neurotoxins produced by the various strains of clostridium botulinum bacterium cuts the SNAREs at quite different sites within its surface.

Feltrup et al. (2018) explain that a section of the targeted site of the SNAREs protein wraps itself around the botulinum enzyme by applying a huge portion of the enzyme for certain interactions. This implies that botulinum toxin can achieve a large interaction site by only using a high level of specificity in just one unit. Biologically, the type of specificity the bacteria uses is usually a simple mechanism using only one protein. This shows how clever the bacterium is to unfold such a complicated mechanism.

Deltombe et al. (2019) stated that botulinum toxin inhibits abnormal muscle contraction, making them less stiff. Once the muscles are stiff, there are the relaxations of wrinkles as they also turn soft.

The above process is effective for patients with frown wrinkles, lines along the eyes, and forehead lines. Therefore, it is necessary to note that the process can’t work for wrinkles due to sun injuries or gravity.

What is Botox?

Botox is a neurotoxin chemical usually obtained from clostridium botulinum. The enzyme is found in lakes, soils, and forests.Botulinum toxin treatments are injectedin small quantities to avoid botulism outbreaks, which normally results when Botox is used in large amounts. Therefore, a patient should not be worried about any side effects of botulinum toxin treatments.The anti-wrinkle treatment has several benefits, from cosmetic to medical appliances.

Who Is the Right Candidate for Botulinum Toxin Injections?

It is crucial to seek your cosmetic practitioner’s advice to ascertain if you are eligible for botulinum toxin injections. Several factors, such as the patient’s age, health status, and projections regarding the results, should be considered before botulinum toxin treatments.

The anti-wrinkle treatment can be done on patients 18 years of age and above.However, the following clients are not eligible for botulinum toxin injections;

  • Nursing
  • Pregnant
  • Have weak facial muscles
  • Have neuromuscular conditions

Botulinum toxin treatments should be doneto treat severe to moderate wrinkles. The patient is also supposed to be realistic when it comes to expectations of the anti-wrinkle treatment.Results vary from one client to the other. It is, therefore, important for patients to consult a cosmetic practitioner to determine if they are the perfect candidates for Botox procedures.

How Long Do Botox Results Last?

Patients will notice some changes2- 3 days after the initial treatment.However, full results are noticeable after two weeks.The results of botulinum toxin injections are temporary; they wear off over time. The results of the anti-wrinkletreatment lastup to six months.Therefore, a patient can go for top-up treatments 3-4 monthsafter the treatment to maintainconsistent results. Failure to go for tip-up treatments, the initial wrinkle texture will reappear.Regular anti-wrinkle treatments train the skin muscles to adhere to relaxations. These treatments cause prolonged results.

Frequently Asked Questions about Botox

Is Botox Safe for Your Health?

Though the anti-wrinkle injection has a toxin substance that leads to botulism, the quantity used is usually in small amounts.The best way to reduce the likelihood of any risks from Botox treatments is by having them done by qualified and skilled cosmetic practitioners.

Is the Second Botox Treatment Quicker Than the First?

Botulinum toxin treatments have the same lasting period.

Are Botox Results Better When Taken Frequently?

Once botulinum toxin treatments are frequently done to the patient, muscles weaken, making the muscles less strong. Therefore, the results tend to last longer since the muscles are trained and retrained, leading to adaptability.

Can You Smile after Botox Treatments?

This relies on how perfectly the chemical was applied to the skin tissue.The sole aim of botulinum toxin procedures is to ensure that patients are left with a rejuvenated and refreshed appearance once treated.Suppose the procedures are done by an inexperienced cosmetic practitioner. In that case, the patient expects incorrect Botox injections, which leave you with frozen appearances on the treated regions.­


For a patient who intends to take botulinum toxin injections for cosmetic procedures, it is good to get a lot of knowledge on how it works.  This view aims to provide the patient with clear and detailed information on how Botox is applied in aesthetic procedures. Therefore, the patient should take more time to determine whether Botox is an ideal option. Patients should remember that Botox results may vary from patient to patient. Therefore, what might have been effective for a certain patient, might not work for the other. It is necessary to consult your cosmetic practitioner as you propose to get anti-wrinkle treatments.


Cracowski, J. L., &Roustit, M. (2020). Human skin microcirculation. Compr. Physiol10(3), 1105-1154.

Deltombe, T., Lejeune, T., &Gustin, T. (2019). Botulinum toxin type A or selective neurotomy for treating focal spastic muscle overactivity?. Annals of physical and rehabilitation medicine62(4), 220-224.

Feltrup, T. M., Patel, K., Kumar, R., Cai, S., & Singh, B. R. (2018). A novel role of the -C-terminus in introducing a functionally flexible structure critical for the biological activity of botulinum neurotoxin. Scientific reports8(1), 1-13.

Jimsheleishvili, S., & Marwaha, K. (2022). Physiology, Neuromuscular Transmission. In StatPearls [Internet]. StatPearls Publishing.

Monika Wasserman